Lipocalin-1 Antibody Pair [HRP]

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Sandwich ELISA: Lipocalin-1 Antibody Pair [HRP] [NBP2-79655] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, S-ELISA
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Standard Curve Range
31.25-2000 pg/ml (example only; lot dependent)
Sensitivity
31.25 pg/ml (example only; lot dependent)

Order Details

Lipocalin-1 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human Lipocalin-1.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Lipocalin-1.
Standard Curve Range
31.25-2000 pg/ml (example only; lot dependent)
Sensitivity
31.25 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Mouse
Gene
LCN1

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot 1:2500-1:10000
Application Notes
1. Capture Antibody: Dilute to a working concentration of 2.0 ug/ml in CBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute standard powder with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 0.15 mg/ml of mouse anti-Lipocalin-1 monoclonal antibody.
  2. Detection Antibody: 0.25 mg/ml mouse anti-Lipocalin-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  3. Standard: Each vial contains 35 ng of recombinant Lipocalin-1.

Alternate Names for Lipocalin-1 Antibody Pair [HRP]

  • LCN1
  • lipocalin 1 (tear prealbumin)
  • lipocalin 1-like 2
  • Lipocalin1
  • Lipocalin-1
  • MGC71975
  • PMFA
  • Tear lipocalin
  • Tear Prealbumin
  • TLC
  • TPVon Ebner gland protein
  • VEG protein
  • VEGP
  • VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol LCN1