Kv1.4 Antibody

Western Blot: Kv1.4 Antibody [NBP2-75552] - Analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Kv1.4 Antibody [NBP2-75552] - Staining Kv1.4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Kv1.4 Antibody [NBP2-75552] - Analysis of paraffin-embedded rat brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.

Flow Cytometry: Kv1.4 Antibody [NBP2-75552] - Analysis of SH-SY5Y cells with Kv1.4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Kv1.4 Antibody [NBP2-75552] - Staining Kv1.4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Kv1.4 Antibody [NBP2-75552] - Analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.

Product Discontinued

Kv1.4 Antibody Summary

• Immunogen: Synthetic peptide within mouse Kv1.4 aa 201-250 / 654. (SwissProt: Q61423 Mouse; SwissProt: P15385 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kv1.4 Antibody

• cardiac potassium channel
• fetal skeletal muscle potassium channel
• HBK4
• HK1
• HPCN2
• HPCN2HUKII
• KCNA4
• KCNA4L
• KCNA8
• Kv1.4
• PCN2
• potassium channel 2
• potassium voltage-gated channel subfamily A member 4
• potassium voltage-gated channel, shaker-related subfamily, member 4
• potassium voltage-gated channel, shaker-related subfamily, member 4-like
• rapidly inactivating potassium channel
• shaker-related potassium channel Kv1.4
• type A potassium channel
• Voltage-gated K(+) channel HuKII
• Voltage-gated potassium channel HBK4
• Voltage-gated potassium channel HK1
• Voltage-gated potassium channel subunit Kv1.4

Background

In neurons voltage-dependent potassium channels are key determinants of the resting membrane potential and of membrane excitability, conditioning the frequency, and shape of action potential. Potassium channels are encoded by numerous genes whose products can be classified into distinct subfamilies. The most distinctively characterized is that of the Kv1 shaker class.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv1.4 Antibody (NBP2-75552)(1)

Publication using NBP2-75552

• Kuijpers M, Kochlamazashvili G, Stumpf A et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum Neuron 2020-10-28 [PMID: 33157003]

Bioinformatics

• Gene Symbol: KCNA4