KPNA5 Overexpression Lysate (Native)

Images

 
Western Blot: KPNA5 Overexpression Lysate (Adult Normal) [NBL1-12373] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KPNA5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KPNA5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208204 and based on accession number NM_002269. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens karyopherin alpha 5 (importin alpha 6) (KPNA5), mRNA.
Gene
KPNA5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
60.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for KPNA5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KPNA5 Overexpression Lysate (Native)

  • importin alpha 6
  • importin subunit alpha-6
  • IPOA6
  • karyopherin alpha 5 (importin alpha 6)
  • Karyopherin subunit alpha-5
  • SRP6

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for KPNA5 Lysate (NBL1-12373) (0)

There are no publications for KPNA5 Lysate (NBL1-12373).
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol KPNA5