KLHL13 Antibody (8D1)

Western Blot: KLHL13 Antibody (8D1) [NBP1-51623] - Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: KLHL13 Antibody (8D1) [NBP1-51623] - Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: KLHL13 Antibody (8D1) [NBP1-51623] - Immunohistochemical analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) using KLHL13 mouse mAb with DAB staining.

Flow Cytometry: KLHL13 Antibody (8D1) [NBP1-51623] - Flow cytometric analysis of 3T3/L1 cells using KLHL13 mouse mAb (green) and negative control (purple).

Product Discontinued

KLHL13 Antibody (8D1) Summary

• Immunogen: Purified recombinant fragment of human KLHL13 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLHL13
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for KLHL13 Antibody (8D1)

• BKLHD2
• BTB and kelch domain containing 2
• BTB and kelch domain-containing protein 2
• FLJ10262
• kelch-like 13 (Drosophila)
• kelch-like protein 13
• KIAA1309
• KLHL13
• MGC74791

Background

Substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex,controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression andcompletion of cytokinesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLHL13