KCNN2 Antibody

Western Blot: KCNN2 Antibody [NBP1-46119] - Staining of human liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: KCNN2 Antibody [NBP1-46119] - Staining of paraffin embedded human kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: KCNN2 Antibody [NBP1-46119] - Staining of paraffin embedded human thyroid gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KCNN2 Antibody Summary

• Immunogen: Peptide with sequence C-ESYDKHVTYNAER corresponding to internal region according to NP_740721.1.
• Epitope: Internal region
• Specificity: This antibody is expected to recognize both isoforms (NP_067627.2; NP_740721.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 70 kDa band and 23 kDa band bands observed in human liver lysates (calculated MW of 63.8 kDa band according to NP_067627.2 and of 26.3 kDa band according to NP_740721.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KCNN2 Antibody

• hSK2
• KCa2.2
• KCa2.2SKCA2
• KCNN2
• potassium intermediate/small conductance calcium-activated channel, subfamilyN, member 2
• SK2apamin-sensitive small-conductance Ca2+-activated potassium channel
• SKCa 2
• SKCa2
• small conductance calcium-activated potassium channel protein 2

Background

Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. This gene is a member of the KCNN family of potassium channel genes. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCNN2 Antibody (NBP1-46119)(1)

Publication using NBP1-46119

• Morimoto T, Ohya S, Hayashi H et al. Cell-cycle-dependent regulation of Ca2+-activated K+ channel in Jurkat T-lymphocyte. J Pharmacol Sci 2007-05-01 [PMID: 17452806]

Bioinformatics

• Gene Symbol: KCNN2
• Entrez:
  • 3781(Human)
  • 140492(Mouse)
  • 54262(Rat)
• Uniprot:
  • NP_067627.2(Human)