Kallikrein 10 Overexpression Lysate (Native)

Images

 
Western Blot: PRSS57 Overexpression Lysate (Adult Normal) [NBL1-12344] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRSS57.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Kallikrein 10 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC218327 and based on accession number NM_002776. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens kallikrein-related peptidase 10 (KLK10), transcript variant 1, mRNA.
Gene
KLK10

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Kallikrein 10

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Kallikrein 10 Overexpression Lysate (Native)

  • breast normal epithelial cell associated serine protease
  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.74
  • Kallikrein 10
  • kallikrein-related peptidase 10
  • KLK10
  • NES1
  • NES1kallikrein-10
  • Normal epithelial cell-specific 1
  • Protease serine-like 1
  • PRSSL1protease, serine-like, 1

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript. All transcript variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Kallikrein 10 Lysate (NBL1-12344) (0)

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Video Protocols

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Bioinformatics

Gene Symbol KLK10
Entrez