Isoleucyl tRNA synthetase Overexpression Lysate (Native)

Images

 
Western Blot: Isoleucyl tRNA synthetase Overexpression Lysate (Adult Normal) [NBP2-10809] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Isoleucyl tRNA ...read more

Product Details

Summary
Applications WB

Order Details

Isoleucyl tRNA synthetase Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_002152. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Isoleucyl tRNA synthetase, mRNA.
Gene
IARS

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
144.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Isoleucyl tRNA synthetase

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Isoleucyl tRNA synthetase Overexpression Lysate (Native)

  • EC 6.1.1
  • EC 6.1.1.5
  • FLJ20736
  • IARS1
  • ILERS
  • ILRS
  • IRS
  • isoleucine tRNA ligase 1, cytoplasmic
  • Isoleucine--tRNA ligase
  • isoleucyl-tRNA synthetase
  • isoleucyl-tRNA synthetase, cytoplasmic
  • PRO0785

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-82001
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB6347
Species: Hu
Applications: WB
MAB2055
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-88650
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22212
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-34136
Species: Hu
Applications: WB, IHC, IHC-P
MAB6777
Species: Hu
Applications: WB, ICC
NBP1-90078
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46403
Species: Hu
Applications: IHC
NBP2-46411
Species: Hu
Applications: WB, IHC
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
MAB18251
Species: Hu, Mu
Applications: WB, Simple Western
NBP2-10809
Applications: WB

Publications for Isoleucyl tRNA synthetase Lysate (NBP2-10809) (0)

There are no publications for Isoleucyl tRNA synthetase Lysate (NBP2-10809).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Isoleucyl tRNA synthetase Lysate (NBP2-10809) (0)

There are no reviews for Isoleucyl tRNA synthetase Lysate (NBP2-10809). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Isoleucyl tRNA synthetase Lysate (NBP2-10809) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Isoleucyl tRNA synthetase Products

Related Products by Gene

Bioinformatics Tool for Isoleucyl tRNA synthetase Lysate (NBP2-10809)

Discover related pathways, diseases and genes to Isoleucyl tRNA synthetase Lysate (NBP2-10809). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Isoleucyl tRNA synthetase Lysate (NBP2-10809)

Discover more about diseases related to Isoleucyl tRNA synthetase Lysate (NBP2-10809).
 

Pathways for Isoleucyl tRNA synthetase Lysate (NBP2-10809)

View related products by pathway.

PTMs for Isoleucyl tRNA synthetase Lysate (NBP2-10809)

Learn more about PTMs related to Isoleucyl tRNA synthetase Lysate (NBP2-10809).
 

Research Areas for Isoleucyl tRNA synthetase Lysate (NBP2-10809)

Find related products by research area.

Blogs on Isoleucyl tRNA synthetase

There are no specific blogs for Isoleucyl tRNA synthetase, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol IARS

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-10809 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Glut4 Antibody
NBP1-49533
Glut4 Antibody
NBP1-49533
Glut4 Antibody
NBP1-49533