Isoleucyl tRNA synthetase Antibody

Product Discontinued

Isoleucyl tRNA synthetase Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IARS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Isoleucyl tRNA synthetase Antibody

• EC 6.1.1
• EC 6.1.1.5
• FLJ20736
• IARS1
• ILERS
• ILRS
• IRS
• isoleucine tRNA ligase 1, cytoplasmic
• Isoleucine--tRNA ligase
• isoleucyl-tRNA synthetase
• isoleucyl-tRNA synthetase, cytoplasmic
• PRO0785

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Isoleucyl tRNA synthetase Antibody (NBP1-87698)(1)

Publication using NBP1-87698

• D'Hulst G, Masschelein E, De Bock K Resistance exercise enhances long-term mTORC1 sensitivity to leucine Molecular metabolism 2022-10-14 [PMID: 36252815] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: IARS