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ING5 Overexpression Lysate

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Western Blot: ING5 Overexpression Lysate (Adult Normal) [NBL1-11993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ING5.
Western Blot: ING5 Overexpression Lysate (Adult Normal) [NBL1-11993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ING5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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ING5 Overexpression Lysate Summary

Description

ING5 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC220333

Accession#: NM_032329

Protein Tag: C-MYC/DDK
Gene
ING5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ING5 Overexpression Lysate

  • inhibitor of growth family, member 5
  • inhibitor of growth protein 5
  • p28ING5FLJ23842

Background

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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HIST1H4F
Overexpression Lysate
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HIST1H4I GST (N-Term) ...
H00008294-P01
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Applications: ELISA, AP, PA, PAGE, WB

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Bioinformatics

Gene Symbol ING5