ING5 Overexpression Lysate (Native)

Western Blot: ING5 Overexpression Lysate (Adult Normal) [NBL1-11993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ING5.

• Reactivity: Hu
• Applications: WB

ING5 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC220333 and based on accession number NM_032329. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens inhibitor of growth family, member 5 (ING5), mRNA.
• Gene: ING5

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 27.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ING5 Overexpression Lysate (Native)

• inhibitor of growth family, member 5
• inhibitor of growth protein 5
• p28ING5FLJ23842

Background

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ING5