ING5 Overexpression Lysate (Native)

Images

 
Western Blot: ING5 Overexpression Lysate (Adult Normal) [NBL1-11993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ING5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ING5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC220333 and based on accession number NM_032329. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens inhibitor of growth family, member 5 (ING5), mRNA.
Gene
ING5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ING5 Overexpression Lysate (Native)

  • inhibitor of growth family, member 5
  • inhibitor of growth protein 5
  • p28ING5FLJ23842

Background

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00051147-B01P
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-P
H00054556-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IF
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF5758
Species: Hu
Applications: WB, IHC, ICC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-37507
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-61159
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-80324
Species: Hu
Applications: WB
NBP2-57745
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-92161
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP2-07996
Species: Hu
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA
NBP2-52487
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
NBP2-56244
Species: Hu
Applications: WB, ICC/IF
NB100-41418
Species: Hu
Applications: WB, IHC, IHC-P, IP
H00008294-P01
Species: Hu

Publications for ING5 Lysate (NBL1-11993) (0)

There are no publications for ING5 Lysate (NBL1-11993).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ING5 Lysate (NBL1-11993) (0)

There are no reviews for ING5 Lysate (NBL1-11993). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ING5 Lysate (NBL1-11993) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for ING5 Lysate (NBL1-11993)

Discover related pathways, diseases and genes to ING5 Lysate (NBL1-11993). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ING5 Lysate (NBL1-11993)

Discover more about diseases related to ING5 Lysate (NBL1-11993).
 

Pathways for ING5 Lysate (NBL1-11993)

View related products by pathway.

PTMs for ING5 Lysate (NBL1-11993)

Learn more about PTMs related to ING5 Lysate (NBL1-11993).
 

Research Areas for ING5 Lysate (NBL1-11993)

Find related products by research area.

Blogs on ING5

There are no specific blogs for ING5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ING5 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ING5