| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04538-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90261-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05519-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06217-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06215-50ul | |||
![Western Blot: ING5 Antibody [NBP3-04538] - Western blot analysis of extracts of 293T cells, using ING5 antibody (NBP3-04538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ING5 Antibody-Western Blot-NBP3-04538-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ING5 Antibody [NBP3-04538] - Western blot analysis of extracts of 293T cells, using ING5 antibody (NBP3-04538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ING5 Antibody [NBP3-04538] - Analysis of MCF-7 cells using ING5 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ING5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04538-img0001.jpg)
![Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of 36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.](http://images.novusbio.com/fullsize/ING5-Antibody-Western-Blot-NB100-1681-img0005.jpg "Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of 36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.")
![Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of 45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time.](http://images.novusbio.com/fullsize/ING5-Antibody-Western-Blot-NB100-1681-img0006.jpg)
![Western Blot: ING5 Antibody (119) [NBP2-90261] - Anti-ING5 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/ING5-Antibody-119-Western-Blot-NBP2-90261-img0002.jpg "Western Blot: ING5 Antibody (119) [NBP2-90261] - Anti-ING5 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: ING5 Antibody (119) [NBP2-90261] - ING5 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-ING5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ING5 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 32 kDa](http://images.novusbio.com/fullsize/ING5-Antibody-119-Immunoprecipitation-NBP2-90261-img0001.jpg)
![Immunohistochemistry-Paraffin: ING5 Antibody [NBP3-05519] - Staining of human thyroid gland shows distinct cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ING5-Antibody-Immunohistochemistry-Paraffin-NBP3-05519-img0001.jpg "Immunohistochemistry-Paraffin: ING5 Antibody [NBP3-05519] - Staining of human thyroid gland shows distinct cytoplasmic and nuclear positivity in glandular cells.")
![Western Blot: ING5 Antibody [NBP1-30999] - ING5 antibody, Internal detects ING5 protein. A. 30 ug Jurkat whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/ING5-Antibody-Western-Blot-NBP1-30999-img0002.jpg "Western Blot: ING5 Antibody [NBP1-30999] - ING5 antibody, Internal detects ING5 protein. A. 30 ug Jurkat whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.")
![Western Blot: ING5 Overexpression Lysate (Adult Normal) [NBL1-11993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ING5.](http://images.novusbio.com/fullsize/ING5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11993-img0002.jpg "Western Blot: ING5 Overexpression Lysate (Adult Normal) [NBL1-11993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ING5.")
![Western Blot: ING5 Antibody [NBP3-06217] - Anti-ING5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell LysateLysatesroteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 110000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDa Observed band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/ING5-Antibody-Western-Blot-NBP3-06217-img0001.jpg "Western Blot: ING5 Antibody [NBP3-06217] - Anti-ING5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell LysateLysatesroteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 110000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDa Observed band size:34 kDa(We are unsure as to the identity of these extra bands.)")