ILF3 Antibody (JG37-63)

Western Blot: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ILF3 Antibody (JG37-63) [NBP2-75540] - Staining ILF3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of paraffin-embedded mouse kidney tissue using anti-ILF3 antibody. Counter stained with hematoxylin.

Flow Cytometry: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of K562 cells with ILF3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ILF3 Antibody (JG37-63) [NBP2-75540] - Staining ILF3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ILF3 Antibody (JG37-63) [NBP2-75540] - Staining ILF3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of paraffin-embedded human tonsil tissue using anti-ILF3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of paraffin-embedded human colon tissue using anti-ILF3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of paraffin-embedded human prostate cancer tissue using anti-ILF3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of paraffin-embedded mouse testis tissue using anti-ILF3 antibody. Counter stained with hematoxylin.

Product Discontinued

ILF3 Antibody (JG37-63) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ILF3 aa 2-51 / 894. (SwissProt: Q12906 Human; SwissProt: Q9Z1X4 Mouse; SwissProt: Q9JIL3 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ILF3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ILF3 Antibody (JG37-63)

• Double-stranded RNA-binding protein 76
• double-stranded RNA-binding protein, 76 kD
• DRBF
• DRBP76M-phase phosphoprotein 4
• dsRNA binding protein NFAR-2/MPP4
• interleukin enhancer binding factor 3, 90kDa
• interleukin enhancer-binding factor 3
• MPHOSPH4interleukin enhancer binding factor 3, 90kD
• MPP4MMP4
• NF90CBTF
• NFAR-1
• NFAR2
• NFARNF110
• NF-AT-90
• Nuclear factor associated with dsRNA
• Nuclear factor of activated T-cells 90 kDa
• nuclear factor of activated T-cells, 90 kD
• TCP110
• TCP80
• Translational control protein 80

Background

ILF3 encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILF3