Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

ELISA: IL-29/IFN-lambda 1 ELISA Kit [NBP1-84819] - Standard curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 2.0 pg/mL

Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric) Summary

• Description: The human IL-29 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-29
• Specificity: The assay detects both natural and recombinant human IL-29/IFNlambda 1. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-29/IFNlambda 1 positive sample. There was no cross-reactivity or interference detected.
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 2.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.1%
• Intra-Assay: CV% < 7.6%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IFNL1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

• cytokine Zcyto21
• IFNL1
• IFN-lambda 1
• IFN-lambda-1
• IL29
• IL-29
• interferon lambda-1
• interferon, lambda 1
• interleukin 29 (interferon, lambda 1)
• interleukin-29
• ZCYTO21

Background

IL-29 is a protein of the helical cytokine family and is a type III interferon sharing many functions with the type I family of interferons. The IL-29 gene is found on chromosome 19 in humans. It is also known as IFN-lambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 does not bind the IFN-alpha/beta receptor, but instead signals through a receptor composed of the IL-28R1 and IL-10R2 subunits. Initial characterizations of the receptor demonstrated that it has a ubiquitous tissue distribution and is expressed on most non-hematopoietic cells. Generation of native IL-29 is achieved by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL 29 has significant antiviral activity and immunoregulatory properties and appears to inhibit T helper-2 (Th2) responses regarding inhibition of IL-13 production, compared with IL-4 or IL-5. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface and the expression of PKR. The ligand/receptor complex seems to signal through the Jak-STAT pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-29/IFN-lambda 1 ELISA Kit (NBP1-84819)(1)

Publication using NBP1-84819

• Burke JM, Gilchrist AR, Sawyer SL, Parker R RNase L limits host and viral protein synthesis via inhibition of mRNA export Science advances 2021-06-01 [PMID: 34088676] (ELISA)

Bioinformatics

• Gene Symbol: IFNL1