Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | containing 172 amino acid residues with two conserved disulfide bonds corresponding to IFN-omega/IFNW1 Source: E. coli Uniprot ID: P05000 Amino Acid Sequence: CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS |
Preparation Method |
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance. |
Details of Functionality | Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | IFNW1 |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of IFN-omega/IFNW1 as determined by LAL method. |
Dilutions |
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Theoretical MW | 20.0 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Research Areas for IFN-omega/IFNW1 Recombinant Protein (NBP2-35893)Find related products by research area.
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Gene Symbol | IFNW1 |