Recombinant Human IFN-omega/IFNW1 Protein

SDS-Page: Human IFN-omega/IFNW1 Protein [NBP2-35893]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IFN-omega/IFNW1 Protein Summary

• Description: containing 172 amino acid residues with two conserved disulfide bonds corresponding to IFN-omega/IFNW1 Source: E. coli

  Uniprot ID: P05000

  Amino Acid Sequence: CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNW1
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IFN-omega/IFNW1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 20.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IFN-omega/IFNW1 Protein

• IFN-omega 1, interferon omega-1
• IFNomega
• IFN-omega
• IFNW1
• Interferon alpha-II-1
• interferon omega-1
• interferon, omega 1

Background

Interferon-Omega (IFN-omega) coded by IFNW1 gene in human, is a number of the type I interferon family, which includes IFN-alpha, IFN-beta, and IFN-omega. The IFNAR-1/IFNAR-2 receptor complex can help with the signal transduction, followed the antiviral or the antiproliferative actions. IFN-omega is derived from IFN-alpha/beta and shares 75 % sequence homology with IFN-alpha. It has two intramolecular disulfide bonds which are crucial for activities. Mire-Sluis et al have described bioassays for IFN-alpha, IFN-beta, and IFN-omega that exploit the ability of these factors to inhibit proliferation of TF-1 cells induced by GM-CSF. The bioassays can be used also with Epo and TF-1 cells, or Epo and Epo-transfected UT-7 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IFNW1