IER2 Antibody

Product Discontinued

IER2 Antibody Summary

• Immunogen: Synthetic peptide, PAKVSRKRRS, representing the internal region of the human protein according to NP_004898.2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IER2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:100-1:2000
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments gave an approx 48kDa band in Human Bone Marrow, Liver, Spleen and Thymus lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.2kDa according to NP_Spleen. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for IER2 Antibody

• ETR101MGC111472
• immediate early response 2
• immediate early response gene 2 protein
• MGC15265
• Protein ETR101

Background

IER2 belongs to a family of immediate early response proteins, and it is thought to play a role in mediating the cellular responses to a variety of extracellular signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IER2
• Entrez:
  • 9592(Human)
  • 52563(Mouse)
  • 291689(Rat)
• Uniprot:
  • NP_004898.2(Human)