HSPB8/HSP22 Overexpression Lysate (Native)

Images

 
Western Blot: Hsp22 Overexpression Lysate (Adult Normal) [NBL1-11761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp22.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HSPB8/HSP22 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201040 and based on accession number NM_014365. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens heat shock 22kDa protein 8 (HSPB8), mRNA.
Gene
HSPB8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HSPB8/HSP22

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HSPB8/HSP22 Overexpression Lysate (Native)

  • Alpha-crystallin C chain
  • CMT2L
  • CRYAC
  • DHMN2
  • E2IG1
  • E2IG1E2-induced gene 1 protein
  • H11
  • H11DHMN2
  • heat shock 22kDa protein 8
  • heat shock 27kDa protein 8
  • heat shock protein beta-8
  • HMN2
  • HMN2A
  • HSP22
  • HSP22CMT2L
  • HSPB8
  • Protein kinase H11
  • Small stress protein-like protein HSP22

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-32972
Species: Hu, Mu, Rt, Ch, Pm, Sh
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
MAB4200
Species: Hu, Mu, Rt
Applications: WB
NBP1-97494
Species: Hu, Mu, Rt, Bv, Ch
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90058
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-07996
Species: Hu
NBP2-52486
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-02459
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
NBP2-38713
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-48588
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-90093
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00027129-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-20154
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-16772
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-06757
Species: Hu
AF4779
Species: Hu
Applications: WB, IHC
AF2584
Species: Mu
Applications: WB, Simple Western

Publications for HSPB8/HSP22 Lysate (NBL1-11761) (0)

There are no publications for HSPB8/HSP22 Lysate (NBL1-11761).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSPB8/HSP22 Lysate (NBL1-11761) (0)

There are no reviews for HSPB8/HSP22 Lysate (NBL1-11761). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSPB8/HSP22 Lysate (NBL1-11761) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HSPB8/HSP22 Products

Bioinformatics Tool for HSPB8/HSP22 Lysate (NBL1-11761)

Discover related pathways, diseases and genes to HSPB8/HSP22 Lysate (NBL1-11761). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HSPB8/HSP22 Lysate (NBL1-11761)

Discover more about diseases related to HSPB8/HSP22 Lysate (NBL1-11761).
 

Pathways for HSPB8/HSP22 Lysate (NBL1-11761)

View related products by pathway.

PTMs for HSPB8/HSP22 Lysate (NBL1-11761)

Learn more about PTMs related to HSPB8/HSP22 Lysate (NBL1-11761).
 

Research Areas for HSPB8/HSP22 Lysate (NBL1-11761)

Find related products by research area.

Blogs on HSPB8/HSP22

There are no specific blogs for HSPB8/HSP22, but you can read our latest blog posts.

Customers Who Bought This Also Bought

HSP27 Antibody (G3.1)
NBP2-32972-0.1mg
H1F0 Antibody
NBP2-16772

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSPB8/HSP22 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol HSPB8
Entrez