HSPB8/HSP22 Antibody (JJ08-53)

Western Blot: HSPB8/HSP22 Antibody (JJ08-53) [NBP2-67836] - Analysis of Hsp22 on different lysates using anti-Hsp22 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Mouse skeletal muscle

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (JJ08-53) [NBP2-67836] - Staining Hsp22 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (JJ08-53) [NBP2-67836] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-Hsp22 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (JJ08-53) [NBP2-67836] - Staining Hsp22 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (JJ08-53) [NBP2-67836] - Staining Hsp22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (JJ08-53) [NBP2-67836] - Analysis of paraffin-embedded mouse brain tissue using anti-Hsp22 antibody. Counter stained with hematoxylin.

Product Discontinued

HSPB8/HSP22 Antibody (JJ08-53) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human HSPB8/HSP22 aa 1-50 / 196. (SwissProt: Q9UJY1 Human; SwissProt: Q9JK92 Mouse; SwissProt: Q9EPX0 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPB8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSPB8/HSP22 Antibody (JJ08-53)

• Alpha-crystallin C chain
• CMT2L
• CRYAC
• DHMN2
• E2IG1
• E2IG1E2-induced gene 1 protein
• H11
• H11DHMN2
• heat shock 22kDa protein 8
• heat shock 27kDa protein 8
• heat shock protein beta-8
• HMN2
• HMN2A
• HSP22
• HSP22CMT2L
• HSPB8
• Protein kinase H11
• Small stress protein-like protein HSP22

Background

HSP22 is encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPB8