HSPB8/HSP22 Antibody (5B12)

Product Discontinued

HSPB8/HSP22 Antibody (5B12) Summary

• Immunogen: HSPB8 (NP_055180, 97 a.a. ~ 197 a.a) partial recombinant protein with GST tag. KVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT
• Specificity: HSPB8 (5B12)
• Isotype: IgG Mix Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPB8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and immunohistochemistry (paraffin).

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSPB8/HSP22 Antibody (5B12)

• Alpha-crystallin C chain
• CMT2L
• CRYAC
• DHMN2
• E2IG1
• E2IG1E2-induced gene 1 protein
• H11
• H11DHMN2
• heat shock 22kDa protein 8
• heat shock 27kDa protein 8
• heat shock protein beta-8
• HMN2
• HMN2A
• HSP22
• HSP22CMT2L
• HSPB8
• Protein kinase H11
• Small stress protein-like protein HSP22

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSPB8/HSP22 Antibody (H00026353-M04)(1)

Publication using H00026353-M04

• Wattin M, Gaweda L, Muller P et al. Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients. Int J Mol Sci 2018-01-07 [PMID: 29316663]

Bioinformatics

• Gene Symbol: HSPB8
• Entrez:
  • 26353(Human)
• OMIM:
  • 158590(Human)
• Uniprot:
  • NP_055180(Human)