HSPB8/HSP22 Antibody (3C12-H11)

Images

 
Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Load: 15 ug protein. Block: 1.5% ...read more
Immunohistochemistry: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:100 for ...read more
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
3C12-H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSPB8/HSP22 Antibody (3C12-H11) Summary

Immunogen
His-tagged human recombinant HSP22 detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB.
Localization
Cytoplasm, Nucleus
Specificity
Detects approx 22kDa. Does not cross react with alpha crystallin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPB8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
1 ug/ml of HSP22 Antibody was sufficient for detection of HSP22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSPB8/HSP22 Antibody (3C12-H11)

  • Alpha-crystallin C chain
  • CMT2L
  • CRYAC
  • DHMN2
  • E2IG1
  • E2IG1E2-induced gene 1 protein
  • H11
  • H11DHMN2
  • heat shock 22kDa protein 8
  • heat shock 27kDa protein 8
  • heat shock protein beta-8
  • HMN2
  • HMN2A
  • HSP22
  • HSP22CMT2L
  • HSPB8
  • Protein kinase H11
  • Small stress protein-like protein HSP22

Background

Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSPB8
Entrez
Uniprot