HspB7 Antibody

Western Blot: HspB7 Antibody [NBP2-99273] - Anti-HspB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart tissue lysate Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa (We are unsure as to the identity of these extra bands).

Immunohistochemistry-Paraffin: HspB7 Antibody [NBP2-99273] - Immunochemical staining HspB7 in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: HspB7 Antibody [NBP2-99273] - Immunochemical staining HspB7 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: HspB7 Antibody [NBP2-99273] - Immunochemical staining HspB7 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: HspB7 Antibody [NBP2-99273] - Immunochemical staining HspB7 in rat heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Immunoprecipitation: HspB7 Antibody [NBP2-99273] - HspB7 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 4 ul anti-HspB7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HspB7 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 22 kDa

Product Discontinued

HspB7 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse HspB7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPB7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000

Reactivity Notes

Cross reactivity in IHC:
Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for HspB7 Antibody

• Cardiovascular heat shock protein
• cvHSP
• DKFZp779D0968
• FLJ32733
• heat shock 27kDa protein family, member 7 (cardiovascular)
• heat shock protein beta-7
• HspB7
• member 7 (cardiovascular)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPB7