HOXA11 Antibody (5A3) - Azide and BSA Free

Western Blot: HOXA11 Antibody (5A3) [H00003207-M08] - Analysis of HOXA11 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: HOXA11 Antibody (5A3) [H00003207-M08] - Analysis of monoclonal antibody to HOXA11 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: HOXA11 Antibody (5A3) [H00003207-M08] - Analysis of HOXA11 expression in Raw 264.7.

ELISA: HOXA11 Antibody (5A3) [H00003207-M08] - Detection limit for recombinant GST tagged HOXA11 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 5A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HOXA11 Antibody (5A3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HOXA11 (NP_005514, 60 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPENLASSDYPGDKSAE
• Specificity: HOXA11 (5A3)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HOXA11
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HOXA11 Antibody (5A3) - Azide and BSA Free

• homeo box A11
• homeobox A11
• Homeobox protein Hox-1I
• homeobox protein HOXA11
• homeobox protein Hox-A11
• HOX1
• HOX1Ihomeo box 1I

Background

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is involved in the regulation of uterine development and is required for female fertility. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXA11 Antibody (H00003207-M08)(1)

Publication using H00003207-M08

• Asfour H, Hirsinger E, Rouco R et al. Inhibitory SMAD6 interferes with BMP dependent generation of muscle progenitor cells and perturbs proximodistal pattern of murine limb muscles Development (Cambridge, England) 2023-05-23 [PMID: 37272529]

Bioinformatics

• Gene Symbol: HOXA11
• Entrez:
  • 3207(Human)
• OMIM:
  • 142958(Human)
• Uniprot:
  • NP_005514(Human)