HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [Allophycocyanin]

Human peripheral blood neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 245106
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
  Met1-Pro363
  Accession # Q8TDS4
• Specificity: Detects human HM74A/PUMA-G/GPR109A. Stains human HM74A/PUMA-G/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: HCAR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [Allophycocyanin]

• GPR109A
• HCA2
• HCAR2
• HM74A
• hydroxycarboxylic acid receptor 2
• Niacr1
• Nicotinic Acid Receptor
• PUMAG

Background

HM74A/GPR109A (also known as PUMA-G in mouse) is a 50-55 kDa 7-transmembrane protein member of a small subfamily within the GPCR #1 family. It is expressed on or in a wide variety of cells, including microglia, neutrophils, islet beta -cells, macrophages, adipocytes, retinal pigment epithelium and keratinocytes. Although HM74A is noted for its ability to bind niacin, it natural ligand is beta -hydroxybutyrate. Thus it may naturally act as a negative regulator of fatty acid release by fat, which is converted to ketone bodies by the liver, and which feedback on adipocytes. Full-length human HM74A shares 95% amino acid (aa) sequence identity with human HM74 and 81% aa sequence identity with mouse HM74A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HCAR2
• Uniprot:
  • Q8TDS4()