Recombinant Human HLA DOA Protein

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SDS-Page: HLA DOA Protein [NBP2-23211]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human HLA DOA Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 26-217 of Human HLA DOA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTKADHMG SYGPAFYQSY GASGQFTHEF DEEQLFSVDL KKSEAVWRLP EFGDFARFDP QGGLAGIAAI KAHLDILVER SNRSRAINVP PRVTVLPKSR VELGQPNILI CIVDNIFPPV INITWLRNGQ TVTEGVAQTS FYSQPDHLFR KFHYLPFVPS AEDVYDCQVE HWGLDAPLLR HWELQVPIPP PDAME

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HLA-DOA
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
24.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human HLA DOA Protein

  • HLA class II histocompatibility antigen, DO alpha chain
  • HLA-D0-alpha
  • HLA-DNAHLADZ
  • HLA-DZA
  • lymphocyte antigen
  • major histocompatibility complex, class II, DN alpha
  • major histocompatibility complex, class II, DO alpha
  • MHC class II antigen DOA
  • MHC DN-alpha
  • MHC DZ alpha

Background

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. Recombinant human HLA-DAO protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol HLA-DOA