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HKDC1 Antibody

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Western Blot: HKDC1 Antibody [NBP2-43539] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody diluted at 1:2000.
Western Blot: HKDC1 Antibody [NBP2-43539] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: HKDC1 Antibody [NBP2-43539] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HKDC1 protein stained by HKDC1 antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: HKDC1 Antibody [NBP2-43539] - Paraffin-embedded mouse duodenum. HKDC1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: HKDC1 Antibody [NBP2-43539] - Paraffin-embedded rat brain. HKDC1 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HKDC1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HKDC1. The exact sequence is proprietary.
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HKDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for HKDC1 Antibody

  • EC 2.7.1
  • EC 2.7.1.1
  • FLJ22761
  • FLJ37767
  • hexokinase domain containing 1
  • Hexokinase domain-containing protein 1
  • MGC125688
  • putative hexokinase HKDC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucokinase antibody [N1C1-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-33144] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glucokinase protein stained by Glucokinase antibody [N1C1-2] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Western Blot: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of Hexokinase 1 on different lysates using anti-Hexokinase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: MCF-7 Lane 4: HepG2
Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Staining Hexokinase 1 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded mouse skin tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
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Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II protein stained by Hexokinase II antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat muscle.Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] - Staining of human bone marrow shows high expression.
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Applications: WB, IHC, IHC-P
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Immunocytochemistry/Immunofluorescence: FLNC Antibody [NBP1-89300] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining shown in green.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HKDC1 Antibody (NBP2-43539) (0)

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Video Protocols

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Bioinformatics

Gene Symbol HKDC1