![HKDC1 antibody [HL3464] detects HKDC1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.HKDC1 stained by HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48816_rabbit-hkdc1-mab-hl3464-immunohistochemistry-paraffin-141120251841295.jpg "HKDC1 antibody [HL3464] detects HKDC1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.HKDC1 stained by HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL3464 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit HKDC1 Antibody (HL3464) (NBP3-48816) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human HKDC1. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HKDC1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HKDC1 |
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48816_rabbit-hkdc1-mab-hl3464-western-blot-141120251839411.jpg "Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![HKDC1 antibody [HL3464] detects HKDC1 protein at mitochondria by immunofluorescent analysis.Sample: AsPC-1 cells were fixed in ice-cold MeOH for 5 min.Green: HKDC1 stained by HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48816_rabbit-hkdc1-mab-hl3464-immunocytochemistry-immunofluorescence-1411202518391019.jpg "HKDC1 antibody [HL3464] detects HKDC1 protein at mitochondria by immunofluorescent analysis.Sample: AsPC-1 cells were fixed in ice-cold MeOH for 5 min.Green: HKDC1 stained by HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48816_rabbit-hkdc1-mab-hl3464-western-blot-141120251843310.jpg "Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48816_rabbit-hkdc1-mab-hl3464-western-blot-14112025184252.jpg "Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry Cadherin-13 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cadherin13_AF3264_Immunohistochemistry_15873.jpg)
![Immunocytochemistry Cadherin-13 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cadherin-13_AF3264_Immunocytochemistry_6491.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")