Histone H3.3B Antibody

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Histone H3.3B antibody (NBP3-48508) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H3.3B antibody (NBP3-48508) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H3.3B antibody (NBP3-48508) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(NBP3-48508) antibody at 1:500 dilution. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis. Sample: Paraffin-embedded mouse spinal cord. Histone H3.3B antibody (NBP3-48508) diluted at 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H3.3B antibody (NBP3-48508) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3B antibody (NBP3-48508) diluted at 1:10000.

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis. Sample: Paraffin-embedded mouse spinal cord. Histone H3.3B antibody (NBP3-48508) diluted at 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3.3B protein stained by Histone H3.3B antibody (NBP3-48508) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.

Histone H3.3B antibody detects H3F3B protein by western blot analysis.A. 50 ug rat brain lysate/extract15% SDS-PAGEHistone H3.3B antibody (NBP3-48508) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE NBP3-48508 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3.3B antibody (NBP3-48508) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H3.3B antibody (NBP3-48508) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H3.3B Antibody Summary

• Description: Novus Biologicals Rabbit Histone H3.3B Antibody (NBP3-48508) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human Histone H3.3B Recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H3F3B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Histone H3.3B Antibody

• H3 histone, family 3A
• H3 histone, family 3B (H3.3B)
• H3.3BH3.3A
• H3F3
• H3F3A
• histone H3.3

Background

Histone H3.3B is 136 amino acids long, weighs approximately 15 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, malaria, leukemia, retinoblastoma, and immunodeficiency. Histone H3.3B has also been shown to have interactions with PELP1, HIST1H4A, HIST1H4B, HIST1H4C, and HIST1H4D in pathways such as the histone modification, development notch signaling, signal transduction for Activin A, amyloids, hemostasis, meiosis, meiotic recombination, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H3F3B