![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0008.jpg "Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, ChIP |
| Clone | ST50-08 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Histone H3.3 Antibody (ST50-08) (NBP2-67530) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Histone H3.3 aa 50-100. (SwissProt: P84243 Human; SwissProt: P84244 Mouse; SwissProt: P84245 Rat) |
| Localization | Nucleus, Chromosome. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H3-3A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.3 Antibody (NBP2-67530)Find related products by research area.
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| Gene Symbol | H3-3A |
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0014.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0007.jpg "Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0002.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0003.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0004.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0005.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0009.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0010.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human lung tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0011.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human lung tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human cervix tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0012.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human cervix tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0013.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin HIST1H3A [ac Lys18] Antibody](https://images.novusbio.com/images/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence HIST1H3A [ac Lys18] Antibody](https://images.novusbio.com/images/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Immunohistochemistry-Paraffin HIST1H3A [ac Lys18] Antibody](https://images.novusbio.com/images/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115031.jpg)
![Knockout Validated Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Knockout_Validated_22540.jpg)
![Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Immunocytochemistry__Immunofluorescence_19067.jpg)
![Western Blot Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Western_Blot_13853.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")