![Western Blot: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - Western Blot of HeLa whole cell lysate, recombinant Histone H3.3, and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.](http://images.novusbio.com/fullsize/nbp3-26046_rabbit-histone-h3-3-mab-rm190-azide-and-bsa-free-2262024231103.png "Western Blot: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - Western Blot of HeLa whole cell lysate, recombinant Histone H3.3, and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.")
| Reactivity | VbSpecies Glossary |
| Applications | WB, ELISA, Func |
| Clone | RM190 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A peptide corresponding to Human Histone H3.3 |
| Specificity | This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H3-3A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.3 Antibody (NBP3-26046)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H3-3A |
![Multiplex Immunoassay: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - RM190 reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.](http://images.novusbio.com/fullsize/nbp3-26046_rabbit-histone-h3-3-mab-rm190-azide-and-bsa-free-24620241245913.png "Multiplex Immunoassay: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - RM190 reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")