![Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202411472125.jpg "Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 28H4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | Synthesized peptide [UniProt P84243] |
| Modification | p Thr3 |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H3-3A |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | H3-3A |
![Immunocytochemistry/Immunofluorescence: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Analysis of Histone H3.3 [p Thr3] Antibody (28H4) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-28220241323624.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Analysis of Histone H3.3 [p Thr3] Antibody (28H4) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Flow Cytometry: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Overlay histogram showing Hela cells stained with Histone H3.3 [p Thr3] Antibody (28H4) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202414183663.jpg "Flow Cytometry: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Overlay histogram showing Hela cells stained with Histone H3.3 [p Thr3] Antibody (28H4) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")