Histone H3.1 Antibody

Images

 
Western Blot: Histone H3.1 Antibody [NB100-81964] - Analysis of extract from HeLa cells using Histone H3.1 (Ab-10) antibody (Lane 1 and 2) and Histone H3.1 (phospho-Ser10) antibody (Lane 3 and 4).
Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NB100-81964] - Staining of methanol-fixed Hela cells using Histone H3.1(Ab-10) antibody
Western Blot: Histone H3.1 Antibody [NB100-81964] - Analysis of extracts from 293 cells using Histone H3.1 (Ab-10) antibody

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Histone H3.1 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around aa. 10 (R-K-S-T-G).
Specificity
Histone H3.1 (specific to a.a. 10) detects endogenous levels of total Histone H3.1 protein. NB100-81964 is also cross-reactive with Histone H3.2 and H3.3 from human mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H3E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3.1 Antibody

  • H3 histone family, member D
  • H3.1HIST1H3D
  • H3/d
  • H3FA
  • H3FB
  • H3FC
  • H3FDHIST1H3F
  • H3FF
  • H3FH
  • H3FI
  • H3FJ
  • H3FK
  • H3FL
  • HIST1H3A
  • HIST1H3B
  • HIST1H3C
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
  • histone 1, H3e
  • histone cluster 1, H3e
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Histone H3.1.

H3.1 - A core histone essential for genome storage and organization
Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t...  Read full blog post.

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Bioinformatics

Gene Symbol HIST1H3E
Entrez
Uniprot