Histone H3.1 Antibody

Western Blot: Histone H3.1 Antibody [NB100-81964] - Analysis of extract from HeLa cells using Histone H3.1 (Ab-10) antibody (Lane 1 and 2) and Histone H3.1 (phospho-Ser10) antibody (Lane 3 and 4).

Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NB100-81964] - Staining of methanol-fixed Hela cells using Histone H3.1(Ab-10) antibody

Western Blot: Histone H3.1 Antibody [NB100-81964] - Analysis of extracts from 293 cells using Histone H3.1 (Ab-10) antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Histone H3.1 Antibody Summary

• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around amino acids 8~12 (R-K-S-T-G).
• Specificity: Detects endogenous levels of total Histone H3.1 protein. NB100-81964 is also cross-reactive with Histone H3.2 and H3.3 from human mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIST1H3E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histone H3.1 Antibody

• H3 histone family, member D
• H3.1HIST1H3D
• H3/d
• H3FA
• H3FB
• H3FC
• H3FDHIST1H3F
• H3FF
• H3FH
• H3FI
• H3FJ
• H3FK
• H3FL
• HIST1H3A
• HIST1H3B
• HIST1H3C
• HIST1H3G
• HIST1H3H
• HIST1H3I
• HIST1H3J
• histone 1, H3e
• histone cluster 1, H3e
• Histone H3.1
• Histone H3/a
• Histone H3/b
• Histone H3/c
• Histone H3/d
• Histone H3/f
• Histone H3/h
• Histone H3/i
• Histone H3/j
• Histone H3/k
• Histone H3/l

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H3E
• Entrez:
  • 8353(Human)
  • 319151(Mouse)
• Uniprot:
  • P68431(Human)