Histone H2B type 1B Antibody

Immunocytochemistry/Immunofluorescence: Histone H2B type 1B Antibody [NBP3-23376] - Immunocytochemical labeling of Histone H2B type 1B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B type 1B (C-terminus) antibody (NBP3-23376), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Western Blot: Histone H2B type 1B Antibody [NBP3-23376] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H2B type 1B Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Histone H2B synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human histone H2B. This sequence is well conserved in rat, mouse, fish, and drosophila histone H2B, and has low homology to other histone family members. [UniProt# P33778]
• Specificity: The antibody detects a 15 kDa protein corresponding to the molecular mass of Histone H2B type 1B on SDS-PAGE immunoblots of human A431, Jurkat, HeLa, mouse C2C12, rat PC12, and A7r5 cells. The antibody also shows nuclear labeling in immunocytochemistry of methanol/acetone fixed cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2BC3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:5000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Histone H2B type 1B Antibody

• H2B histone family, member F
• H2B/fHistone H2B.f
• H2BFFH2B.1
• histone 1, H2bb
• histone cluster 1, H2bb
• histone H2B type 1-B
• Histone H2B.1
• MGC119804

Background

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BC3