Histone H2B type 1B Antibody (SR1212) - BSA Free

Immunohistochemistry: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21687.

Western Blot: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Western blot analysis using NBP3-21687 in Hela cell treated with TSA lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP, ChIP
• Clone: SR1212
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2B type 1B Antibody (SR1212) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Histone H2B (Uniprot #: P33778)
• Specificity: Detects endogenous levels of Histone H2B type 1B
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H2BC3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Flow Cytometry 1:10
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:10
  • Western Blot 1:5000-1:20000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H2B type 1B Antibody (SR1212) - BSA Free

• H2B histone family, member F
• H2B/fHistone H2B.f
• H2BFFH2B.1
• histone 1, H2bb
• histone cluster 1, H2bb
• histone H2B type 1-B
• Histone H2B.1
• MGC119804

Background

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BC3