Histone H2B type 1B [ac Lys20] Antibody (21F11) - BSA Free

Western Blot: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30 minutes. All lanes: Histone H2B type 1B antibody at 0.135ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa.

Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30 minutes) with at Histone H2B type 1B antibody 1:84, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Overlay histogram showing Hela cells stained with Histone H2B type 1B antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunocytochemistry analysis of Histone H2B type 1B antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 21F11
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2B type 1B [ac Lys20] Antibody (21F11) - BSA Free Summary

• Description: Novus Biologicals Rabbit Histone H2B type 1B [ac Lys20] Antibody (21F11) - BSA Free (NBP3-15229) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide. UniProt ID (P33778).
• Modification: ac Lys20
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H2BC3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:500
  • Immunocytochemistry/ Immunofluorescence 1:30-1:200
  • Western Blot 1:5000-1:10000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H2B type 1B [ac Lys20] Antibody (21F11) - BSA Free

• H2B histone family, member F
• H2B/fHistone H2B.f
• H2BFFH2B.1
• histone 1, H2bb
• histone cluster 1, H2bb
• histone H2B type 1-B
• Histone H2B.1
• MGC119804

Background

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BC3