Home » Histone H1.2 » Histone H1.2 Antibodies » Histone H1.2 Antibody

Histone H1.2 Antibody

 Submit a Review

Images

 
(
Enlarge)
Immunohistochemistry: Histone H1.2 Antibody [NBP2-49202] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemistry: Histone H1.2 Antibody [NBP2-49202] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

Histone H1.2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KKAGATAGKRKASGPPVSELITKAVAA
Predicted Species
Mouse (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H1C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Histone H1.2 Recombinant Protein Antigen (NBP2-49202PEP)

Reactivity Notes

Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Histone H1.2 Antibody

  • H1 histone family, member 2
  • H1.2
  • H1F2H1C
  • histone 1, H1c
  • histone cluster 1, H1c
  • histone H1.2
  • Histone H1d
  • MGC3992

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The Histone H1.2 protein is 213 amino acids long, 21 kDA and is encoded by the HIST1H1C gene. H1 proteins are essential in forming chromatin fiber and for the condensation of nucleosome chains into higher-order structured fibers. The HIST1H1C gene has been investigated in leukemia and parkinson's disease. It plays a role in apoptosis, PKA signaling, cell cycle chromosome condensation in prometaphase, and histone modification through interactions with various genes such as TONSL, NPM1, YWHAQ, EED, and ESR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Histone H1
Antibody (HH1/957)
NBP2-45184
Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)
(1 Review)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Histone H1.4
Antibody
NBP2-16847
Western Blot: Histone H1.4 Antibody [NBP2-16847] - Sample (20 ug of cell lysate ) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Histone H1.4 Antibody [NBP2-16847] - Hela xenograft, using Histone H1.4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu
Applications: IHC, IHC-P, WB
Histone H1.3
Overexpression Lysate
NBL1-11554
Western Blot: Histone H1.3 Overexpression Lysate (Adult Normal) [NBL1-11554] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H1.3.
Species: Hu
Applications: WB
Histone H1.5
Antibody
NBP3-17162
Immunohistochemistry-Paraffin: Histone H1.5 Antibody [NBP3-17162] - Staining of human tonsil shows strong nuclear positivity in germinal- and non germinal center cells.
Species: Hu
Applications: IHC, IHC-P
Histone H1T
Antibody
NBP2-56042
Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human testis shows nuclear positivity in ductus seminiferous.
Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIST1H1T antibody NBP2-56042 (A) shows similar protein distribution across tissues to independent antibody NBP2-57668 (B).
Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human kidney.
Species: Hu
Applications: IHC, IHC-P
Tau [p Thr181]
Antibody
NB100-82245
Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Western blot analysis of extract from mouse brain tissue using Tau (Ab-181) antibody ( Lane 1 and 2) and Tau (phospho-Thr181) antibody ( Lane 3 and 4).
Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Thr181) Antibody and the same antibody preincubated with blocking peptide.
Western Blot: Tau [p Thr181] Antibody [NB100-82245] - PPP2R2C level regulates Tau phosphatase levels. Representative image of immunoblots of indicated antibodies are shown in SHSY5Y cell lines after knockdown and overexpression PPP2R2C. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103048) licensed under a CC-BY licence.
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, KD, Simple Western, WB
BAK Antibody
(SU32-07)
NBP2-67460
Flow Cytometry: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.
(1 Publication)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
H2A Antibody (4C10)
H00008337-M01
Immunocytochemistry/Immunofluorescence: H2A Antibody (4C10) [H00008337-M01] - Analysis of monoclonal antibody to HIST2H2AA3 on HeLa cell . Antibody concentration 10 ug/ml.
Species: Hu
Applications: ELISA, ICC/IF, WB
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2) - BSA Free
NBP2-52486
ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
H1F0 Antibody
(1415-1) - Azide and ...
NBP2-47752
Flow Cytometry: H1F0 Antibody (1415-1) - Azide and BSA Free [NBP2-47752] - Flow Cytometric Analysis of HeLa cells using H1F0 antibody (1415-1) - Azide and BSA Free conjugated to goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) - Azide and BSA Free [NBP2-47752] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (1415-1) - Azide and BSA Free
Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) - Azide and BSA Free [NBP2-47752] - Human Tonsil stained with Histone H1 Monoclonal antibody (1415-1)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, WB
YB1 Antibody
NBP1-89945
Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Histamine H4R
Antibody - BSA Free
NLS3775
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma
(1 Publication)
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
(34 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CCDC6 Antibody
NBP1-85351
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human fallopian tube shows high expression.
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Histone H2a
Antibody
NB100-56346
Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.
Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
(1 Review)
(3 Publications)
Species: Hu, Mu, Sh
Applications: ELISA, Flow, IHC, IHC-P, WB

Publications for Histone H1.2 Antibody (NBP2-49202) (0)

There are no publications for Histone H1.2 Antibody (NBP2-49202).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Histone H1.2 Antibody (NBP2-49202) (0)

There are no reviews for Histone H1.2 Antibody (NBP2-49202). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Histone H1.2 Antibody (NBP2-49202) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Histone H1.2 Products

Bioinformatics Tool for Histone H1.2 Antibody (NBP2-49202)

Discover related pathways, diseases and genes to Histone H1.2 Antibody (NBP2-49202). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Histone H1.2 Antibody (NBP2-49202)

Discover more about diseases related to Histone H1.2 Antibody (NBP2-49202).
 

Pathways for Histone H1.2 Antibody (NBP2-49202)

View related products by pathway.

Research Areas for Histone H1.2 Antibody (NBP2-49202)

Find related products by research area.

Blogs on Histone H1.2

There are no specific blogs for Histone H1.2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Histone H1.2 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol HIST1H1C