HIST1H3A [p Ser10] Antibody (rPHH3/6824) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Applications: IHC
• Clone: rPHH3/6824
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

HIST1H3A [p Ser10] Antibody (rPHH3/6824) [Alexa Fluor™ Plus 680] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10) (Uniprot: P68431)
• Modification: p Ser10
• Localization: Nucleus, Chromosome
• Marker: Nuclear Marker
• Specificity: Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H3C1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HIST1H3A [p Ser10] Antibody (rPHH3/6824) [Alexa Fluor™ Plus 680]

• H3 histone family, member A
• H3/A
• H3FAHIST1H3E
• H3FB
• H3FC
• H3FD
• H3FF
• H3FH
• H3FI
• H3FJ
• H3FK
• H3FL
• HIST1H3B
• HIST1H3C
• HIST1H3D
• HIST1H3F
• HIST1H3G
• HIST1H3H
• HIST1H3I
• HIST1H3J
• histone 1, H3a
• histone cluster 1, H3a
• histone H3.1
• Histone H3/a
• Histone H3/b
• Histone H3/c
• Histone H3/d
• Histone H3/f
• Histone H3/h
• Histone H3/i
• Histone H3/j
• Histone H3/k
• Histone H3/l

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H3C1