HINFP Antibody

Western Blot: HINFP Antibody [NB100-56628] - Analysis of HINFP in MOLT-4 cell lysate using this antibody at 1 ug/ml.

Product Discontinued

HINFP Antibody Summary

• Immunogen: This antibody was developed against a mixture of synthetic peptides corresponding to amino acid residues, 180-194, 331-346, and 371-388 of human MIZF.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HINFP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for HINFP Antibody

• HiNF-PDKFZP434F162
• histone H4 gene-specific protein HiNF-P
• histone H4 transcription factor
• Histone nuclear factor P
• MBD2 (methyl-CpG-binding protein)-interacting zinc finger protein
• MBD2-interacting zinc finger 1
• MBD2-interacting zinc finger protein
• Methyl-CpG-binding protein 2-interacting zinc finger protein
• MIZFDKFZp434F162
• ZNF743MBD2-interacting zinc finger

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. Recently, using MBD2 as bait in two-hybrid screening, a novel zing finger protein, called MIZF has been identified. This MBD2-binding zinc finger (MIZF) protein represses transcription by associating with MBD2 and a histone deacetylase complex. It is ubiquitously expressed in all human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HINFP
• Uniprot:
  • Q9BQA5(Human)