hHpr1-p84-Thoc1 Antibody (JU53-18)

Western Blot: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Analysis of Nuclear Matrix Protein p84 on different lysates using anti-Nuclear Matrix Protein p84 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: A431Lane 3: Mouse skeletal muscle Lane 4: PC-12

Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Staining Nuclear Matrix Protein p84 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Analysis of paraffin-embedded rat brain tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Staining Nuclear Matrix Protein p84 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Analysis of paraffin-embedded human colon cancer tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Analysis of paraffin-embedded human tonsil tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Analysis of paraffin-embedded human lung cancer tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (JU53-18) [NBP2-75521] - Analysis of paraffin-embedded rat testis tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.

Product Discontinued

hHpr1-p84-Thoc1 Antibody (JU53-18) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Nuclear Matrix Protein p84 aa 367-416 / 657. (SwissProt: Q96FV9 Human; SwissProt: Q8R3N6 Mouse; SwissProt: P59924 Rat)
• Localization: SwissProt: Q96FV9(Human) Q8R3N6(Mouse) P59924(Rat)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: THOC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for hHpr1-p84-Thoc1 Antibody (JU53-18)

• HPR1THO complex subunit 1
• hTREX84
• Nuclear matrix protein p84
• P84
• P84N5
• THO complex 1
• tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THOC1