H60 Antibody (205326) [Unconjugated]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse H60 Monoclonal Antibody (Catalog # MAB1155, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
• Clone: 205326
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

H60 Antibody (205326) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with mouse H60 and mouse myeloma cell line NS0-derived recombinant mouse H60
  Asp30-Gln212
  Accession # Q3TDZ7
• Specificity: Detects mouse H60 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: H60a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 Not Assigned
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse H60 Biotinylated Antibody (Catalog number BAF1155)
  Standard: Recombinant Mouse H60 Fc Chimera (Catalog number 1155-H6)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for H60 Antibody (205326) [Unconjugated]

• H60
• H60a

Background

H60 was originally described as an immunodominant histocompatibility antigen that is expressed in BALB mice but not in B6 mice. More recently it was reported to function as a ligand for mouse NKG2D, an activating receptor found on NK cells, on some T cell subsets, and on stimulated macrophages. H60 shares approximately 25 percent amino acid identity with the Rae-1 family, a small group of proteins that also function as ligands for mouse NKG2D. H60 and the Rae-1 proteins are distantly related to MHC class I proteins, but they possesses only the alpha 1 and alpha 2 Ig-like domains, and have no capacity to bind peptide or interact with beta 2‑microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. Unlike the GPI-linked Rae-1 proteins, H60 appears to be anchored to the membrane via a hydrophobic transmembrane segment. H60 transcripts were found in embryonic tissue, in spleen, and in some transformed cell lines. Transcripts were also observed in mouse skin cells after exposure to carcinogens. Binding of H60 to NKG2D results in the activation of cytolytic activity and/or cytokine production by the NKG2D-expressing effector cells. Ectopic expression of H60 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑6).

1. Malarkannan, S. et al. (1998) J. Immunol. 161:3501.
2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
3. Cerwenka, A. et al. (2000) Immunity 12:721.
4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
5. Diefenbach, A. et al. (2001) Nature 413:165.
6. NKG2D and its Ligands, www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for H60 Antibody (MAB1155)(8)

Publications using MAB1155

• A Dabla, YC Liang, N Rajabalee, C Irwin, CGJ Moonen, JV Willis, S Berton, J Sun TREM2 Promotes Immune Evasion by Mycobacterium tuberculosis in Human Macrophages MBio, 2022-08-04;13(4):e0145622. 8/4/2022 [PMID: 35924849]
• Y Kanda, A Takeuchi, M Ozawa, Y Kurosawa, T Kawamura, D Bogdanova, H Iioka, E Kondo, Y Kitazawa, H Ueta, K Matsuno, T Kinashi, T Katakai Visualizing the Rapid and Dynamic Elimination of Allogeneic T Cells in Secondary Lymphoid Organs J. Immunol., 2018-06-20;0(0):. 2018-06-20 [PMID: 29925676] (Flow Cytometry, Mouse)
• Li Hua, Mingli Fang, Boqi Dong, Sheng Guo, Cuiyun Cui, Jiwei Liu et al. Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice Oncotarget 2016-12-13 [PMID: 27448968]
• NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo J Immunol, 2016-05-02;196(11):4805-13. 2016-05-02 [PMID: 27183590] (Flow Cytometry, Mouse)
• Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015-01-29;135(5):1311-9. 2015-01-29 [PMID: 25634359] (Flow Cytometry, Western Blot, Mouse)
• Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 2009-02-06;113(24):6120-7. 2009-02-06 [PMID: 19202127] (Flow Cytometry, Mouse)
• Timothy J. Nice, Laurent Coscoy, David H. Raulet Posttranslational regulation of the NKG2D ligand Mult1 in response to cell stress Journal of Experimental Medicine 2009-02-16 [PMID: 19171762] (Flow Cytometry, Mouse)
• Gourzi P, Leonova T, Papavasiliou FN A role for activation-induced cytidine deaminase in the host response against a transforming retrovirus. Immunity, 2006-06-01;24(6):779-86. 2006-06-01 [PMID: 16782033] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: H60a
• Uniprot:
  • Q3TDZ7()