H60 Antibody (205326) [Phycoerythrin]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse H60 PE-conjugated Monoclonal Antibody (Catalog # FAB1155P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 205326
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

H60 Antibody (205326) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with mouse H60 and mouse myeloma cell line NS0-derived recombinant mouse H60
  Asp30-Gln212
  Accession # Q3TDZ7
• Specificity: Detects mouse H60 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: H60a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for H60 Antibody (205326) [Phycoerythrin]

• H60
• H60a

Background

H60 was originally described as an immunodominant histocompatibility antigen that is expressed in BALB mice but not in B6 mice. More recently it was reported to function as a ligand for mouse NKG2D, an activating receptor found on NK cells, on some T cell subsets, and on stimulated macrophages. H60 shares approximately 25 percent amino acid identity with the Rae-1 family, a small group of proteins that also function as ligands for mouse NKG2D. H60 and the Rae-1 proteins are distantly related to MHC class I proteins, but they possesses only the alpha 1 and alpha 2 Ig-like domains, and have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. Unlike the GPI-linked Rae-1 proteins, H60 appears to be anchored to the membrane via a hydrophobic transmembrane segment. H60 transcripts were found in embryonic tissue, in spleen, and in some transformed cell lines. Transcripts were also observed in mouse skin cells after exposure to carcinogens. Binding of H60 to NKG2D results in the activation of cytolytic activity and/or cytokine production by the NKG2D-expressing effector cells. Ectopic expression of H60 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1-6).

1. Malarkannan, S. et al. (1998) J. Immunol. 161:3501.
2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
3. Cerwenka, A. et al. (2000) Immunity 12:721.
4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
5. Diefenbach, A. et al. (2001) Nature 413:165.
6. NKG2D and its Ligands, www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for H60 Antibody (FAB1155P)(7)

Publications using FAB1155P

• José A. Casado, Antonio Valeri, Rebeca Sanchez-Domínguez, Paula Vela, Andrea López, Susana Navarro et al. Upregulation of NKG2D ligands impairs hematopoietic stem cell function in Fanconi anemia Journal of Clinical Investigation 2022-08-01 [PMID: 35671096]
• L Ma, M Han, Z Keyoumu, H Wang, S Keyoumu Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma Med. Sci. Monit., 2017-04-25;23(0):1980-1991. 2017-04-25 [PMID: 28439064] (Flow Cytometry, Mouse)
• Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation. Nat Neurosci, 2016-01-11;19(2):243-52. 2016-01-11 [PMID: 26752157] (Flow Cytometry, Mouse)
• Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity. Leukemia, 2013-11-13;28(6):1316-25. 2013-11-13 [PMID: 24336127] (Flow Cytometry, Mouse)
• Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells. J. Immunol., 2012-06-29;189(3):1162-72. 2012-06-29 [PMID: 22753933] (Flow Cytometry, Mouse)
• Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection. Infect. Immun., 2011-12-05;80(2):565-74. 2011-12-05 [PMID: 22144492] (Flow Cytometry, Mouse)
• Walsh KB, Lanier LL, Lane TE NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis. J. Virol., 2007-12-26;82(6):3031-44. 2007-12-26 [PMID: 18160433] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: H60a
• Uniprot:
  • Q3TDZ7()