H1F0 Antibody (SPM256) - IHC-Prediluted

Immunohistochemistry-Paraffin: H1F0 Antibody (SPM256) - IHC-Prediluted [NBP2-48058] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with H1F0 Antibody (SPM256).

Immunohistochemistry-Paraffin: H1F0 Antibody (SPM256) - IHC-Prediluted [NBP2-48058] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 Antibody (SPM256)

Immunohistochemistry-Paraffin: H1F0 Antibody (SPM256) - IHC-Prediluted [NBP2-48058] - Human Tonsil stained with Histone H1 Monoclonal Antibody (SPM256)

Western Blot Analysis of human heart tissue lysate using H1F0 Antibody (SPM256) - IHC-Prediluted.

Flow Cytometric Analysis of HeLa cells. H1F0 Antibody (SPM256) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Confocal Immunofluorescence image of HeLa cells using H1F0 Antibody (SPM256) - IHC-Prediluted (green-CF488). Phalloidin is used to label the membranes.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: SPM256
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

H1F0 Antibody (SPM256) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Nuclei of human leukemia biopsy cells
• Localization: Nuclear
• Marker: Pan Nuclear Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H1-0
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Hu-chromosome location: 3q21-q22; 6q21-q22; 22q13.1 Molecular weight of antigen: ~30kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for H1F0 Antibody (SPM256) - IHC-Prediluted

• H1 histone family, member 0
• H1.0, H1(0), H1-0
• H10
• H1FVMGC5241
• Histone H1'
• Histone H1(0)
• histone H1.0

Background

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-0