H1F0 Antibody (rAE-4) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: rAE-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

H1F0 Antibody (rAE-4) [DyLight 650] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human H1F0 protein
• Localization: Nuclear
• Marker: Pan Nuclear Marker
• Specificity: Please note that this antibody is a recombinant Mouse version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 monoclonal antibody. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a purer preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H1-0
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for H1F0 Antibody (rAE-4) [DyLight 650]

• H1 histone family, member 0
• H1.0, H1(0), H1-0
• H10
• H1FVMGC5241
• Histone H1'
• Histone H1(0)
• histone H1.0

Background

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-0