GSPT1 Antibody (CL13336) - BSA Free

Western Blot: GSPT1 Antibody (CL13336) [NBP3-18559] - Analysis in human cell line K562.

Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of A-431 cells using the Anti-GSPT1 monoclonal antibody, showing specific staining in cytosol in green. Nuclear probes are visualized in blue (where available).

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human liver shows no positivity in hepatocytes.

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human cervical cancer shows strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human colorectal cancer (metastasis to ovary) shows strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human colorectal cancer shows moderate to strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL13336
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GSPT1 Antibody (CL13336) - BSA Free Summary

• Immunogen: This antibody was developed using a synthetic peptide derived from P15170, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSPT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for GSPT1 Antibody (CL13336) - BSA Free

• ERF3A
• eRF3aFLJ38048
• ETF3A
• eukaryotic peptide chain release factor GTP-binding subunit ERF3A
• Eukaryotic peptide chain release factor subunit 3a
• FLJ39067
• G1 to S phase transition 1,551G9.2
• G1 to S phase transition protein 1 homolog
• GST1

Background

GSPT1 is involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates theactivity of ERF1. Involved in regulation of mammalian cell growth

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSPT1