GRK3 Antibody (497018) [mFluor Violet 610 SE]

• Reactivity: Hu
• Applications: WB
• Clone: 497018
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

GRK3 Antibody (497018) [mFluor Violet 610 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: E. coli-derived recombinant human GRK3
  Pro469-Leu688
  Accession # P35626
• Specificity: Detects endogenous human GRK3 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRK3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product MAB4785. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GRK3 Antibody (497018) [mFluor Violet 610 SE]

• ADRBK2
• adrenergic, beta, receptor kinase 2
• BARK2
• BARK2beta-adrenergic receptor kinase 2
• Beta-ARK-2
• EC 2.7.11
• EC 2.7.11.15
• G-protein-coupled receptor kinase 3
• GRK3
• GRK3Beta-ARK-2

Background

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second essenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of beta-adrenergic receptor kinase, GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK3