GRK1 Antibody (G8) - BSA Free

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a Monclonal Antibody (G8) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - illustrates immunolocalization of GRK1a in bovine retina

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a Monclonal Antibody (G8) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a Monclonal Antibody (G8) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ICC/IF, IP
• Clone: G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GRK1 Antibody (G8) - BSA Free Summary

• Immunogen: Full length human GRK1.
• Epitope: This antibody recognizes an epitope in the C-terminal region of GRK1a.
• Specificity: Detects G-protein-associated rhodopsin kinase 1a (GRK1a).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2 - 3 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: WB: Detects an approx. 60 kDa protein representing GRK1a from bovine rod outer segments. IF: Staining of GRK1a in bovine retina with ab2775 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GRK1 Antibody (G8) - BSA Free

• G protein-coupled receptor kinase 1rhodopsin kinase
• GPRK1
• GRK1
• RHOK
• RHOKEC 2.7.11
• RKEC 2.7.11.14

Background

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK1
• Entrez:
  • 6011(Human)
  • 24013(Mouse)
  • 81760(Rat)