GRK1 Antibody (G8) - BSA Free

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Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 ...read more
Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - illustrates immunolocalization of GRK1a in bovine retina
Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 ...read more
Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (G8) [NB120-2775] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IP
Clone
G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

GRK1 Antibody (G8) - BSA Free Summary

Immunogen
Full length human GRK1.
Epitope
This antibody recognizes an epitope in the C-terminal region of GRK1a.
Specificity
Detects G-protein-associated rhodopsin kinase 1a (GRK1a).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GRK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2 - 3 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 60 kDa protein representing GRK1a from bovine rod outer segments. IF: Staining of GRK1a in bovine retina with ab2775 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GRK1 Antibody (G8) - BSA Free

  • G protein-coupled receptor kinase 1rhodopsin kinase
  • GPRK1
  • GRK1
  • RHOK
  • RHOKEC 2.7.11
  • RKEC 2.7.11.14

Background

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ch
Applications: WB, ICC/IF, IP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GRK1
Entrez