GRK1 Antibody (D11) [Alexa Fluor™ Plus 555]

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF, IHC, IP
• Clone: D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

GRK1 Antibody (D11) [Alexa Fluor™ Plus 555] Summary

• Immunogen: Full length human GRK1.
• Epitope: This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
• Specificity: Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This does not cross-react with other G protein-coupled kinases.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GRK1 Antibody (D11) [Alexa Fluor™ Plus 555]

• G protein-coupled receptor kinase 1rhodopsin kinase
• GPRK1
• GRK1
• RHOK
• RHOKEC 2.7.11
• RKEC 2.7.11.14

Background

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK1