GPR50 Antibodies
We offer GPR50 Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Western Blot. Each GPR50 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GPR50 Antibodies can be used in a variety of model species: Canine, Human, Monkey, Sheep. Use the list below to choose the GPR50 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GPR50 polyclonal antibodies and browse our GPR50 monoclonal antibody catalog.
Alternate Names for GPR50 Antibodies
anti-GPR50 antibody, anti-G protein-coupled receptor 50H9Mel1c antibody, anti-melatonin-related receptor antibody, anti-MGC125342 antibody
6 Results for "gpr50" in Primary Antibodies
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TVRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TVRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: GPR50 Antibody [H00009248-B01P] - Analysis of negative control 293 cells (Black) and GPR50 expressing 293 cells (Green) using GPR50 purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/GPR50-Antibody-Flow-Cytometry-H00009248-B01P-img0003.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - Analysis of GPR50 expression in transfected 293T cell line by GPR50 polyclonal antibody. Lane 1: GPR50 transfected lysate(67.87 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 promotes ligand-independent activation of TBRI signaling. Detection of p-Smad3, p-Stat-3, p-Jak2 (c) and p-Smad2 (d) in lysates of hypothalamus and cortex of wt and GPR50 ko mice. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TBRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0006.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009248-b01p_mouse-polyclonal-gpr50-antibody-310202415482257.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009248-b01p_mouse-polyclonal-gpr50-antibody-310202415482280.jpg)
![Immunohistochemistry-Paraffin: GPR50 Antibody [NLS751] - Analysis of anti-GPR50 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NLS751-img0003.jpg "Immunohistochemistry-Paraffin: GPR50 Antibody [NLS751] - Analysis of anti-GPR50 antibody with human brain, glioblastoma.")
![Immunohistochemistry-Paraffin: GPR50 Antibody [NLS751] - Analysis of anti-GPR50 antibody with human brain, hypothalamus at 10 ug/ml.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NLS751-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GPR50 Antibody [NBP2-56258] - Staining of human cell line HEK 293 shows localization to plasma membrane. Antibody staining si shown in green.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56258-img0001.jpg "Immunocytochemistry/Immunofluorescence: GPR50 Antibody [NBP2-56258] - Staining of human cell line HEK 293 shows localization to plasma membrane. Antibody staining si shown in green.")
![Immunohistochemistry-Paraffin: GPR50 Antibody [NBP2-56258] - Staining of human anterior pituitary gland shows strong cytoplasmic positivity in endocrine cells.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NBP2-56258-img0005.jpg)
![Immunohistochemistry-Paraffin: GPR50 Antibody [NLS6618] - Analysis of anti-GPR50 antibody with human pituitary.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NLS6618-img0004.jpg "Immunohistochemistry-Paraffin: GPR50 Antibody [NLS6618] - Analysis of anti-GPR50 antibody with human pituitary.")
![Immunohistochemistry-Paraffin: GPR50 Antibody [NBP2-14069] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NBP2-14069-img0001.jpg "Immunohistochemistry-Paraffin: GPR50 Antibody [NBP2-14069] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.")
![Immunohistochemistry-Paraffin: GPR50 Antibody [NBP2-14069] - Staining of human anterior pituitary gland shows strong cytoplasmic positivity in endocrine cells.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NBP2-14069-img0002.jpg)
![Western Blot: GPR50 Antibody [NBP1-98295] - Titration: 1.0 ug/ml Positive Control: Jurkat Whole Cell.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-NBP1-98295-img0002.jpg "Western Blot: GPR50 Antibody [NBP1-98295] - Titration: 1.0 ug/ml Positive Control: Jurkat Whole Cell.")