GPR35 Antibody (1007216) [Unconjugated]

Western blot shows lysates of human ileum tissue and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR35 Monoclonal Antibody (Catalog # MAB10181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GPR35 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1007216
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR35 Antibody (1007216) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human GPR35
  Accession # Q9HC97
• Specificity: Detects human GPR35 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR35 Antibody (1007216) [Unconjugated]

• G protein-coupled receptor 35
• GPR35
• G-protein coupled receptor 35
• KYNA receptor
• Kynurenic acid receptor

Background

Human G protein-coupled receptor 35 also known as GPR35 (also known as Kynurenic acid or KYNA receptor) is a 7-transmembrane domain G protein-coupled receptor encoded by the GPR35 gene. Polymorphisms in the G protein–coupled receptor GPR35 are associated with increased risk for certain inflammatory diseases of the bile duct and large intestine that have increased cancer risk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9HC97()