GPR17 Antibody

Western Blot: GPR17 Antibody [NBP1-51988] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer) with antibody at 3 ug/mL. Detected by chemiluminescence. Approx 40 kDa band observed in Mouse Brain lysates (calculated MW of 37.8kDa according to Mouse NP_001020552.1 ).

Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NBP1-51988] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NBP1-51988] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GPR17 Antibody Summary

• Immunogen: This GPR17 Antibody was develped against a peptide with sequence C-EGKTNESSLSAKSE corresponding to C-Terminus according to NP_005282.1.
• Epitope: C-EGKTNESSLSAKSE
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPR17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 2 - 3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR17 Antibody

• DKFZp686M18273
• G protein-coupled receptor 17
• GPR17
• G-protein coupled receptor 17
• P2Y-like Receptor
• R12
• UDP/CysLT Receptor
• uracil nucleotide/cysteinyl leukotriene receptor

Background

GPR17, a former orphan G-coupled protein is specifically activated by P2Y and CysLT endogenous ligands, leading to both adenylyl cyclase inhibition and intracellular calcium increases. In both rats and humans GPR17 expressed in the organs which suffer ischemic damage, like the brain, heart and kidneys. Studies by Ciana, et. al. have shown that inhibition of GPR17 reduces ischemic damage in a rat focal ischemia model. These results suggest GPR17 is the common molecular target mediating brain damage by nucleotides and CysLTs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR17 Antibody (NBP1-51988)(1)

Publication using NBP1-51988

• Parravicini C, Ranghino G, Abbracchio MP, Fantucci P. GPR17: molecular modeling and dynamics studies of the 3-D structure and purinergic ligand binding features in comparison with P2Y receptors. BMC Bioinformatics 2008-06-04 [PMID: 18533035]

Bioinformatics

• Gene Symbol: GPR17
• Entrez:
  • 2840(Human)
  • 574402(Mouse)
  • 767613(Rat)
• Uniprot:
  • NP_005282.1(Human)