GNAI3 Antibody - BSA Free

Western Blot: GNAI3 Antibody [NBP1-32617] - Wild-type (WT) and G protein alpha Inhibitor 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha Inhibitor 3 antibody [N2C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: GNAI3 Antibody [NBP1-32617] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: GNAI3 Antibody [NBP1-32617] - SAS xenograft, using G protein alpha Inhibitor 3 antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: GNAI3 Antibody [NBP1-32617] - (30 ug whole cell lysate)- A: A431 B: MOLT4 C: Raji 7.5% SDS PAGE diluted at 1:1000

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [N2C3] (NBP1-32617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [N2C3] (NBP1-32617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Gp, RM, Xp, Ze
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GNAI3 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit GNAI3 Antibody - BSA Free (NBP1-32617) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GNAI3. The exact sequence is proprietary.
• Predicted Species: Rat (97%), Porcine (99%), Bovine (98%), Guinea Pig (97%), Rhesus Macaque (100%), Zebrafish (94%), Chicken (97%), Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAI3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Japanese Medaka (91%), Xenopus laevis (94%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GNAI3 Antibody - BSA Free

• FLJ26559
• G(i) alpha-3
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6
• guanine nucleotide-binding protein G(k) subunit alpha

Background

GAI3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor regulated K(+) channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GNAI3