GNAI3 Antibody (HL2096) - Azide and BSA Free

Knockout Validated: GNAI3 Antibody (HL2096) - Azide and BSA Free [NBP3-25487] - Wild-type (WT) and GNAI3 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: GNAI3 Antibody (HL2096) - Azide and BSA Free [NBP3-25487] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GNAI3 Antibody (HL2096) - Azide and BSA Free [NBP3-25487] - GNAI3 antibody [HL2096] detects GNAI3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GNAI3 stained by GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GNAI3 Antibody (HL2096) - Azide and BSA Free [NBP3-25487] - GNAI3 antibody [HL2096] detects GNAI3 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GNAI3 stained by GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: GNAI3 Antibody (HL2096) - Azide and BSA Free [NBP3-25487] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: GNAI3 Antibody (HL2096) - Azide and BSA Free [NBP3-25487] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GNAI3 antibody [HL2096] detects GNAI3 protein by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: GNAI3 stained by GNAI3 antibody [HL2096] (NBP3-25487) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL2096
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GNAI3 Antibody (HL2096) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit GNAI3 Antibody (HL2096) - Azide and BSA Free (NBP3-25487) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human GNAI3
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GNAI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GNAI3 Antibody (HL2096) - Azide and BSA Free

• FLJ26559
• G(i) alpha-3
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6
• guanine nucleotide-binding protein G(k) subunit alpha

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in varioustransmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAI3