GCC185 Antibody

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - (3.8 ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GCC185 Antibody Summary

• Immunogen: Peptide with sequence C-QKLTNKNNKIED corresponding to internal region according to NP_055450.1, NP_852118.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GCC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 60 kDa band in human lymph node and testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185 kDa band according to NP_055450.1 and of 196 kDa band according to NP_852118.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Paraffin embedded Human Spleen, Small Intestine and Kidney.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCC185 Antibody

• 185 kDa Golgi coiled-coil protein
• CLL-associated antigen KW-11
• CTCL tumor antigen se1-1
• GCC protein, 185-kD
• GCC185GRIP and coiled-coil domain-containing 2
• Golgi coiled-coil protein GCC185
• GRIP and coiled-coil domain containing 2
• GRIP and coiled-coil domain-containing protein 2
• KIAA0336REN53
• Ran-binding protein 2-like 4
• RANBP2L4
• Renal carcinoma antigen NY-REN-53

Background

The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCC185 Antibody (NBP1-20922)(1)

Publication using NBP1-20922

• Efimov A, Kharitonov A, Efimova N et al. Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Dev Cell 2007-06-01 [PMID: 17543864]

Bioinformatics

• Gene Symbol: GCC2
• Entrez:
  • 9648(Human)
• Uniprot:
  • NP_055450.1(Human)
  • NP_852118.1(Human)
  • Q8IWJ2(Human)