GCC185 Antibody

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Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - (3.8 ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GCC185 Antibody Summary

Immunogen
Peptide with sequence C-QKLTNKNNKIED corresponding to internal region according to NP_055450.1, NP_852118.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GCC2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human lymph node and testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185 kDa band according to NP_055450.1 and of 196 kDa band according to NP_852118.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human spleen shows select staning of splenocytes.
Publications
Read Publication using NBP1-20922.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCC185 Antibody

  • 185 kDa Golgi coiled-coil protein
  • CLL-associated antigen KW-11
  • CTCL tumor antigen se1-1
  • GCC protein, 185-kD
  • GCC185GRIP and coiled-coil domain-containing 2
  • Golgi coiled-coil protein GCC185
  • GRIP and coiled-coil domain containing 2
  • GRIP and coiled-coil domain-containing protein 2
  • KIAA0336REN53
  • Ran-binding protein 2-like 4
  • RANBP2L4
  • Renal carcinoma antigen NY-REN-53

Background

The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCC185 Antibody (NBP1-20922)(1)

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Bioinformatics

Gene Symbol GCC2
Entrez
Uniprot